Product Pathways - MAPK Signaling
Phospho-MAPKAPK-2 (Thr334) Antibody #3041
PhosphoSitePlus® protein, site, and accession data: MAPKAPK2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R Mk | Endogenous | 49 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3041:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
Phospho-MAPKAPK-2 (Thr334) Antibody detects endogenous levels of MAPKAPK-2 protein phosphorylated at threonine 334. It may also cross-react with MAPKAPK-3 phosphorylated at threonine 313.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from untreated or UV treated HeLa and Cos cells, using Phospho-MAPKAPK-2 (Thr334) Antibody (upper) or MAPKAPK-2 Antibody #3042 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing, nuclear localization, using Phospho-MAPKAPK-2 (Thr334) Antibody.
Background
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
Application References
- Chang, E. et al. (2011) Blood 117, 2527-37. Applications: Western Blotting
- Manke, I.A. et al. (2005) Mol Cell 17, 37-48. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3042 MAPKAPK-2 Antibody
- 3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
