Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Bub3 Antibody #3049

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Hm Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Bub3 Antibody detects endogenous levels of total Bub3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human Bub3.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Bub3 Antibody.

Background

The Mitotic Checkpoint Complex (MCC) , which contains Bub1, Bub1b, Bub3, Mad2 and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions. (1). This complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C) during mitosis to prevent cells with unaligned chromosomes from prematurely entering anaphase (2). Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6) and is required for functional microtubule-kinetochore interactions (7).

  1. Fukagawa, T. (2008) Front Biosci 13, 2705-13.
  2. Chen, R.H. (2007) J Biomed Sci 14, 475-9.
  3. Dai, W. et al. (2004) Cancer Res 64, 440-5.
  4. Kops, G.J. et al. (2005) Nat Rev Cancer 5, 773-85.
  5. Baker, D.J. et al. (2004) Nat Genet 36, 744-9.
  6. Taylor, S.S. et al. (1998) J Cell Biol 142, 1-11.
  7. Logarinho, E. et al. (2008) Mol Biol Cell 19, 1798-813.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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