Cell Signaling Technology

Product Pathways - Metabolism

LKB1 (27D10) Rabbit mAb #3050

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 54 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LKB1 (27D10) Rabbit mAb detects endogenous levels of LKB1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LKB1.

Western Blotting

Western Blotting

Western blot analysis of extracts from Caki-1 and COS cells using LKB1 (27D10) Rabbit mAb.

IP

IP

Immunoprecipitation of LKB1 from insulin treated 293T cell extracts using LKB1 (27D10) antibody (Lane1). Lane 2: No antibody control. Lane 3: Input control.

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, 
including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

  1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
  2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
  3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
  4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
  5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
  6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
  7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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