Product Pathways - Glucose Metabolism
LKB1 (27D10) Rabbit mAb #3050
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H R Mk | Endogenous | 54 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
LKB1 (27D10) Rabbit mAb detects endogenous levels of LKB1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human LKB1.
Background
Peutz-Jeghers syndrome (PJS) is caused by mutation of the tumor suppressor kinase LKB1 (1,2). The amino-terminal noncatalytic domain of LKB1 comprises both a nuclear localization signal (3) and a putative cytoplasmic retention signal (4). LKB1 is phosphorylated in cells at Ser31, 325 and 431 and at Thr189, 336 and 366 (5,6). LKB1 activates AMP-activated protein kinase (AMPK) via phosphorylation at Thr172 (7-9).AMPK plays a key role in the regulation of energy homeostasis, sensing conditions of low ATP and signaling to downstream effector molecules that conserve ATP and regulate cell growth and apoptosis.
- Hemminki, A. et al. (1998) Nature 391, 184-187.
- Jenne, D.E. et al. (1998) Nat. Genet. 18, 38-43.
- Smith, D.P. et al. (1999) Hum. Mol. Genet. 8, 1479-1485.
- Nezu, J. et al. (1999) Biochem. Biophys. Res. Commun. 261, 750-755.
- Sapkota, G.P. et al. (2001) J. Biol. Chem. 276, 19469-19482.
- Sapkota, G.P. et al. (2002) Biochem. J. 362, 481-490.
- Hawley, S.A. et al. (2003) J.Biol. 2, 28.
- Woods, A. et al. (2003) Curr. Biol. 13, 2004-2008.
- Shaw, R.J. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335.
Application References
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Companion Products
- 3047 LKB1 (D60C5) Rabbit mAb
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- 3054 Phospho-LKB1 (Thr189) Antibody
- 3055 Phospho-LKB1 (Ser334) Antibody
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.