Cell Signaling Technology

Product Pathways - Glucose Metabolism

LKB1 (27D10) Rabbit mAb #3050

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H R Mk Endogenous 54 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

LKB1 (27D10) Rabbit mAb detects endogenous levels of LKB1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human LKB1.

Western Blotting

Western Blotting

Western blot analysis of extracts from Caki-1 and COS cells using LKB1 (27D10) Rabbit mAb.

IP

IP

Immunoprecipitation of LKB1 from insulin treated 293T cell extracts using LKB1 (27D10) antibody (Lane1). Lane 2: No antibody control. Lane 3: Input control.

Background

Peutz-Jeghers syndrome (PJS) is caused by mutation of the tumor suppressor kinase LKB1 (1,2). The amino-terminal noncatalytic domain of LKB1 comprises both a nuclear localization signal (3) and a putative cytoplasmic retention signal (4). LKB1 is phosphorylated in cells at Ser31, 325 and 431 and at Thr189, 336 and 366 (5,6). LKB1 activates AMP-activated protein kinase (AMPK) via phosphorylation at Thr172 (7-9).AMPK plays a key role in the regulation of energy homeostasis, sensing conditions of low ATP and signaling to downstream effector molecules that conserve ATP and regulate cell growth and apoptosis.

  1. Hemminki, A. et al. (1998) Nature 391, 184-187.
  2. Jenne, D.E. et al. (1998) Nat. Genet. 18, 38-43.
  3. Smith, D.P. et al. (1999) Hum. Mol. Genet. 8, 1479-1485.
  4. Nezu, J. et al. (1999) Biochem. Biophys. Res. Commun. 261, 750-755.
  5. Sapkota, G.P. et al. (2001) J. Biol. Chem. 276, 19469-19482.
  6. Sapkota, G.P. et al. (2002) Biochem. J. 362, 481-490.
  7. Hawley, S.A. et al. (2003) J.Biol. 2, 28.
  8. Woods, A. et al. (2003) Curr. Biol. 13, 2004-2008.
  9. Shaw, R.J. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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