Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

NTF2 (5A3) Mouse mAb #3053

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk Endogenous 14 Mouse IgG2a

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NTF2 (5A3) Mouse mAb detects endogenous levels of total NTF2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length recombinant human NTF2.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using NTF2 (5A3) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using NTF2 (5A3) Mouse mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).

Background

The small GTPase Ran resides on both the cytosolic and nucleosolic sides of the nuclear pore complex (NPC) and regulates the import and export of various proteins to and from the nucleus. Like other small GTPases, Ran exists in either a GTP-bound or GDP-bound state. RanGTP that resides in the nucleus and promotes nuclear export, while cytosolic RanGDP promotes import. The gradient of RanGTP across the nuclear membrane allows for appropriate movement of cargo proteins across the NPC as well as maintenance of the mitotic spindle (1-3).Nuclear transport factor 2 (NTF2) regulates the subcellular distribution and function of Ran (4-5). The NTF2 homodimer facilitates the diffusion of RanGDP through NPCs via transient interactions with phenylalanine-glycine (FG) repeat domains on NPC proteins. NTF2 stabilizes the GDP-bound form of Ran until it is induced to dissociate by a nuclear factor in an ATP-dependent manor, thus allowing the guanine nucleotide exchange factor (GEF) RCC1 to mediate exchange of GDP for GTP on Ran (6-7).

  1. Mattaj, I.W. and Englmeier, L. (1998) Annu Rev Biochem 67, 265-306.
  2. Kalab, P. et al. (2002) Science 295, 2452-6.
  3. Becskei, A. and Mattaj, I.W. (2003) Proc Natl Acad Sci USA 100, 1717-22.
  4. Ribbeck, K. et al. (1998) EMBO J 17, 6587-98.
  5. Steggerda, S.M. et al. (2000) Mol Biol Cell 11, 703-19.
  6. Stewart, M. (2000) Cell Struct Funct 25, 217-25.
  7. Yamada, M. et al. (2004) J Biol Chem 279, 36228-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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