Cell Signaling Technology

Product Pathways - Metabolism

Phospho-LKB1 (Thr189) Antibody #3054

Applications Reactivity Sensitivity MW (kDa) Source
W H Transfected Only 54 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-LKB1 (Thr189) Antibody detects transfected levels of LKB1 only when phosphorylated at threonine 189. The antibody does not cross-react with LKB1 phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr189 of human LKB1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with Wild-type LKB1, LKB1 (T189A), LKB1 (S334A) or LKB1 (S428A), using Phospho-LKB1 (Thr189) Antibody .

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, 
including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

  1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
  2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
  3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
  4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
  5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
  6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
  7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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