Cell Signaling Technology

Product Pathways - Glucose Metabolism

Phospho-LKB1 (Thr189) Antibody #3054

Applications Reactivity Sensitivity MW (kDa) Source
W H Transfected Only 54 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-LKB1 (Thr189) Antibody detects transfected levels of LKB1 only when phosphorylated at threonine 189. The antibody does not cross-react with LKB1 phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr189 of human LKB1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with Wild-type LKB1, LKB1 (T189A), LKB1 (S334A) or LKB1 (S428A), using Phospho-LKB1 (Thr189) Antibody .

Background

Peutz-Jeghers syndrome (PJS) is caused by mutation of the tumor suppressor kinase LKB1 (1,2). The amino-terminal noncatalytic domain of LKB1 comprises both a nuclear localization signal (3) and a putative cytoplasmic retention signal (4). LKB1 is phosphorylated in cells at Ser31, 325 and 431 and at Thr189, 336 and 366 (5,6). LKB1 activates AMP-activated protein kinase (AMPK) via phosphorylation at Thr172 (7-9).AMPK plays a key role in the regulation of energy homeostasis, sensing conditions of low ATP and signaling to downstream effector molecules that conserve ATP and regulate cell growth and apoptosis.

  1. Hemminki, A. et al. (1998) Nature 391, 184-187.
  2. Jenne, D.E. et al. (1998) Nat. Genet. 18, 38-43.
  3. Smith, D.P. et al. (1999) Hum. Mol. Genet. 8, 1479-1485.
  4. Nezu, J. et al. (1999) Biochem. Biophys. Res. Commun. 261, 750-755.
  5. Sapkota, G.P. et al. (2001) J. Biol. Chem. 276, 19469-19482.
  6. Sapkota, G.P. et al. (2002) Biochem. J. 362, 481-490.
  7. Hawley, S.A. et al. (2003) J.Biol. 2, 28.
  8. Woods, A. et al. (2003) Curr. Biol. 13, 2004-2008.
  9. Shaw, R.J. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 3329-3335.

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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