Cell Signaling Technology

Product Pathways - MAPK Signaling

MKP3 Antibody #3058

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 48 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MKP3 Antibody detects endogenous levels of total MKP3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MKP3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from SKMEL5, HepG2 and COS cells using MKP3 Antibody.

Background

MAP kinases are inactivated by dual-specificity protein phosphatases known as MAPK phosphatases (MKP), that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. MKPs specifically dephosphorylate both threonine and tyrosine residues in MAPK P-loops and have been shown to play important roles in regulating the function of the MAPK family (1,2). At least 13 members of the family (MKP-1, PAC-1, VHR, MKP-2, HVH3, MKP-3, PYST2, HVH5, MKP-4, MKP-5, MKP-6, MKP-7, DSP2) display unique substrate specificities for various MAP kinases (3). MAPK phosphatases typically contain an amino-terminal rhodanese-fold responsible for MKP docking to MAPK family members and a carboxy-terminal catalytic domain (4). These phosphatases can play important roles in development, immune system function, stress responses and metabolic homeostasis (5), and also in the development of cancer and the response of cancer cells to chemotherapy (6).

MKP3 specifically dephosphorylates ERK MAP kinase (7).

  1. Camps, M. et al. (2000) FASEB J 14, 6-16.
  2. Theodosiou, A. and Ashworth, A. (2002) Genome Biol 3, REVIEWS3009.
  3. Salojin, K. and Oravecz, T. (2007) J Leukoc Biol 81, 860-9.
  4. Tanoue, T. et al. (2002) J Biol Chem 277, 22942-9.
  5. Dickinson, R.J. and Keyse, S.M. (2006) J Cell Sci 119, 4607-15.
  6. Wu, G.S. (2007) Cancer Metastasis Rev 26, 579-85.
  7. Kim, Y. et al. (2003) Biochemistry 42, 15197-207.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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