Cell Signaling Technology

Product Pathways - Protein Folding

HSPB8/HSP22 Antibody #3059

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 22 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HSPB8/HSP22 Antibody detects endogenous levels of total HSPB8/HSP22 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSPB8/HSP22. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HSPB8/HSP22 Antibody.

Background

HSPB8 (HSP22) is a member of the small heat shock protein superfamily and the human protein is most closely related to HSP27. Similar to most other small HSPs (sHSPs), HSPB8 is predominantly transcribed in skeletal muscle and heart (1). In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N- and C-terminal) that are specific for different binding partners, and has the ability to bind to itself and other sHSPs such as HSPB7 and HSPB2 (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macroautophagy (4).

  1. Kappé, G. et al. (2001) Biochim Biophys Acta 1520, 1-6.
  2. Benndorf, R. et al. (2001) J Biol Chem 276, 26753-61.
  3. Sun, X. et al. (2004) J Biol Chem 279, 2394-402.
  4. Kim, M.V. et al. (2004) Biochem Biophys Res Commun 325, 649-52.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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