Product Pathways - Tyrosine Kinase / Adaptors
c-Kit (D13A2) XP® Rabbit mAb #3074
|3074S||100 µl (10 western blots)||---||In Stock||---|
|3074P||40 µl (4 western blots)||---||In Stock||---|
|3074||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||120 and 145||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
c-Kit (D13A2) XP® Rabbit mAb detects endogenous levels of total c-Kit protein. It does not cross-react with other receptor tyrosine kinase family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Tyr703 of human c-Kit.
Western blot analysis of extracts from NCI-H526 cells using c-Kit (D13A2) XP® Rabbit mAb.
c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
- Martin, F.H. et al. (1990) Cell 63, 203-11.
- Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
- Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
- Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
- Nocka, K. et al. (1990) EMBO J 9, 1805-13.
- Hirota, S. et al. (1998) Science 279, 577-80.
- Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
- Chen, Y. et al. (2011) J Vis Exp , . Applications: IF-P (paraffin).
- Bosbach, B. et al. (2012) Proc Natl Acad Sci U S A 109, E2276-83. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.