Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Ros (Tyr2274) Antibody #3078

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 258 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Ros (Tyr2274) Antibody detects endogenous levels of Ros only when phosphorylated at tyrosine 2274. This antibody may cross-react with other unknown proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr2274 of human Ros.

Western Blotting

Western Blotting

Western blot analysis of HCC78 cell lysate untreated (upper), or treated with calf intestinal phosphatase (CIP) (middle and lower), using Phospho-Ros (Tyr2274) Antibody (upper and middle), or Ros control antibody (lower). HCC78 cells express a SLC3482-Ros fusion protein which contains an activated Ros kinase fragment. (Rikova, K et al (2007) Cell 131:1190-1203)

Background

The c-ros proto-oncogene encodes a receptor tyrosine kinase similar to insulin receptor proteins and expressed in specific epithelial cells (1). During mouse development the Ros receptor plays an important role in the differentiation of epididymal epithelium (2). Overexpression or mutation of Ros may lead to oncogenic development. Furthermore, stable transfection with a chimeric receptor containing the Ros transmembrane and cytoplasmic domains leads to transformation of NIH/3T3 fibroblasts (3). A genetic rearrangement that produces a fusion protein containing Ros and FIG (fused in glioblastoma) results in glioblastoma formation (4). The FIG-Ros fusion abnormally activates the SHP-2 phosphatase and PI3K/Akt/mTOR pathways in tumor cells (5). Additional Ros mutations are associated with NSCLC cancer cells indicating that Ros may be a candidate for tumor targeted therapy (6).

  1. Keilhack, H. et al. (2001) J Cell Biol 152, 325-34.
  2. Yeung, C.H. et al. (1998) J Reprod Fertil Suppl 53, 137-47.
  3. Riethmacher, D. et al. (1994) Oncogene 9, 3617-26.
  4. Charest, A. et al. (2003) Genes Chromosomes Cancer 37, 58-71.
  5. Charest, A. et al. (2006) Cancer Res 66, 7473-81.
  6. Rikova, K. et al. (2007) Cell 131, 1190-203.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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