Product Pathways - Cell Cycle / Checkpoint
Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079
PhosphoSitePlus® protein, site, and accession data: AurA
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H | Endogenous | 48 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb detects endogenous levels of Aurora A only when phosphorylated at Thr288.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr288 of human Aurora A.
Western Blotting
Western blot analysis of extracts from hydroxyurea or nocodazole treated HeLa and HT29 cells using Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of mitotic HeLa cells during metaphase (left) and anaphase (right) using Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb (red) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).
- Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
- Zhou, H. et al. (1998) Nat Genet 20, 189-93.
- Sen, S. et al. (1997) Oncogene 14, 2195-200.
- Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
- Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
- Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
Application References
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Companion Products
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
