Cell Signaling Technology

Product Pathways - Metabolism

Phospho-C/EBPbeta (Thr235) Antibody #3084

Applications Reactivity Sensitivity MW (kDa) Source
W H M (B) (Pg) Endogenous 19 LIP. 36 LAP. 38 LAP. Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-C/EBPbeta (Thr235) Antibody detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37. This antibody does not cross-react with other phosphorylated C/EBPs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding threonine 235 of human C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from adipocytes (differentiated 3T3-L1) treated with insulin for the indicated times, using Phospho-C/EBPbeta (Thr235) Antibody.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).

  1. Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
  2. Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
  3. Wegner, M. et al. (1992) Science 256, 370-373.
  4. Trautwein, C. et al. (1993) Nature 364, 544-547.
  5. Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
  6. Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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