Product Pathways - Metabolism
PASK (C70B2) Rabbit mAb #3086
|3086S||100 µl (10 western blots)||---||In Stock||---|
|3086||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||170||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
PASK (C70B2) Rabbit mAb detects endogenous levels of total PASK protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse PASK.
PASK is a serine/threonine kinase that contains two PAS (Per-Arnt-Sim) domains (1). Its kinase activity is up-regulated by autophosphorylation of the activation loop within its catalytic domain and is inhibited in cis by one of the PAS domains (1). Studies found that the yeast homolog of PASK phosphorylates and inhibits two enzymes required for glycogen biosynthesis: UDP-glucose pyrophosphorylase and glycogen synthase, resulting in the decrease of carbohydrate storage (2). Further studies showed that increased glucose levels activate PASK activity and enhance its expression in pancreatic β-cells (3). PASK is essential for the glucose-stimulated expression of preproinsulin and Pdx1, suggesting its role in the regulation of genes involved in pancreatic β-cell functions (3). PASK was shown to be critical for glucose-stimulated insulin secretion in pancreatic β-cells (4). The absence of PASK also protects animals from obesity and insulin resistance when they are fed a high-fat diet (4). These findings suggest that PASK functions as an important metabolic sensor in various cells (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.