Cell Signaling Technology

Product Pathways - Glucose Metabolism

C/EBPβ (LAP) Antibody #3087

Applications Reactivity Sensitivity MW (kDa) Source
W H M (R) Endogenous 35 to 38 mouse LAP. 45 to 49 human LAP. Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

C/EBPbeta (LAP) Antibody detects endogenous levels of total C/EBPbeta, the p38 and p36 LAPs, but not the p20 LIP. This antibody does not cross-react with C/EBPalpha, -delta, -gamma, -epsilon or -zeta.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with human or mouse C/EBPbeta (LAP), using C/EBPbeta (LAP) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3-L1, differentiated for the indicated times, using C/EBPbeta (LAP) Antibody.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).

  1. Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
  2. Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
  3. Wegner, M. et al. (1992) Science 256, 370-373.
  4. Trautwein, C. et al. (1993) Nature 364, 544-547.
  5. Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
  6. Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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