Cell Signaling Technology

Product Pathways - Metabolism

IRS-2 (L1326) Antibody #3089

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 185 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IRS-2 (L1326) Antibody detects endogenous levels of total IRS-2 protein. It does not cross-react with IRS-1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1326 of human IRS-2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of H-4-II-E and HeLa cells using IRS-2 (L1326) Antibody.

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

  1. Sun, X.J. et al. (1991) Nature 352, 73-77.
  2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
  3. Withers, D.J. et al. (1998) Nature 391, 900-904.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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