Product Pathways - Tyrosine Kinase / Adaptors
Pyk2 (H364) Antibody #3090
PhosphoSitePlus® protein, site, and accession data: Pyk2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M | Endogenous | 116 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Pyk2 (H364) Antibody detects endogenous levels of total Pyk2 protein. This antibody does not cross-react with other related proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His364 of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat and Ramos cells using Pyk2 (H364) Antibody.
Background
Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).
- Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
- Lev, S. et al. (1995) Nature 376, 737-745.
- Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
- Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
- Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
- Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
- Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3291 Phospho-Pyk2 (Tyr402) Antibody
- 3292 Pyk2 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
