Product Pathways - Cell Cycle / Checkpoint
Aurora A/AIK Antibody #3092
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M Mk | Endogenous | 48 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Aurora A/AIK Antibody detects endogenous levels of total Aurora A/AIK protein. The antibody may cross-react with AIK3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the amino terminus of human Aurora A/AIK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, HepG2 and COS cells, using Aurora A/AIK Antibody.
Western Blotting
Western blot analysis of purified mouse His-Aurora A/AIK expressed in Sf9 insect cells, untreated or treated with okadaic acid 1 hour prior to harvest, using Aurora A/AIK Antibody.
Background
Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6).
- Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
- Zhou, H. et al. (1998) Nat Genet 20, 189-93.
- Sen, S. et al. (1997) Oncogene 14, 2195-200.
- Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
- Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
- Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4718 Aurora A/AIK (1G4) Rabbit mAb
- 3094 Aurora B/AIM1 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
