Product Pathways - Cell Cycle / Checkpoint
Aurora B/AIM1 Antibody #3094
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP F | H M R Mk | Endogenous | 40 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 3094:
- Flow, Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Aurora B/AIM1 Antibody detects endogenous levels of Aurora B/AIM1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human Aurora B/AIM1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Hela, NIH/3T3 and C6 cells, untreated or nocodazole-treated (50 ng/ml, 24 hrs), using Aurora B/AIM1 Antibody.
Flow Cytometry
Flow cytometric analysis of Hela cells, using Aurora B/AIM1 Antibody (blue) compared to a nonspecifc negative control antibody (red).
Background
Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6).
- Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
- Zhou, H. et al. (1998) Nat Genet 20, 189-93.
- Sen, S. et al. (1997) Oncogene 14, 2195-200.
- Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
- Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
- Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2914 Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP™ Rabbit mAb
- 3079 Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb
- 4718 Aurora A/AIK (1G4) Rabbit mAb
- 3092 Aurora A/AIK Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
