Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb #3097
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (M) | Transfected Only | 135 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb detects transfected levels of Ack1 only when phosphorylated at Tyr857/858. This antibody may cross-react with other tyrosine-phosphorylated tyrosine kinases.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr857/858 of human Ack1.
Western Blotting
Western blot analysis of cell extracts from 293 cells, untransfected or transfected with Myc-tagged human Ack1 expression constructs, using Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb (upper and middle) or Ack1 Antibody #3131 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. The cell lysates of 293 cells transfected with Myc-tagged human Ack1 were kindly provided by Dr. Wannian Yang.
Background
Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).
- Galisteo, M.L. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9796-9801.
- Yokoyama, N. and Miller, W.T. (2003) J. Biol. Chem. 278, 47713-47723.
- Yang, W. and Cerione, R.A. (1997) J. Biol. Chem. 272, 24819-24824.
- Yang, W. et al. (2001) J. Biol. Chem. 276, 43987-43993.
- Mahajan, N.P. et al. (2005) Cancer Res. 65, 10514-10523.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4570 TNK1 (C44F9) Rabbit mAb
- 3131 Ack1 Antibody
- 3138 Phospho-Ack1 (Tyr284) Antibody
- 3137 Phospho-Ack1 (Tyr857/858) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
