Product Pathways - Chromatin Regulation / Epigenetics
JMJD1B (C6D12) Rabbit mAb #3100
|3100S||100 µl (10 western blots)||---||In Stock||---|
|3100||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||220||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
JMJD1B (C6D12) Rabbit mAb detects endogenous levels of JMJD1B protein (all three isoforms). This antibody does not cross react with other Jumonji C proteins, including HR, JMJD1A and JMJD1C.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of the human JMJD1B protein.
Western blot analysis of extracts from HeLa and NCCIT cell lines using JMJD1B (C6D12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using JMJD1B (C6D12) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using JMJD1B (C6D12) Rabbit mAb.
The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen and thyroid receptors and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8).
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- Loh, Y.H. et al. (2007) Genes Dev 21, 2545-57.
- Ko, S.Y. et al. (2006) Cell Struct Funct 31, 53-62.
- Hu, Z. et al. (2001) Oncogene 20, 6946-54.
- Lee, J.W. et al. (1995) Mol Endocrinol 9, 243-54.
- Wolf, S.S. et al. (2007) Arch Biochem Biophys 460, 56-66.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.