Product Pathways - TGF-beta/Smad Signaling
Smad2 (L16D3) Mouse mAb #3103
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PhosphoSitePlus® protein, site, and accession data: Smad2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 60 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3103:
- Western Blotting
Specificity / Sensitivity
Smad2 (L16D3) Mouse mAb detects endogenous levels of total Smad2 protein and may detect Smad3 due to high sequence homology.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Smad2 protein.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, COS, and PC12 cells, using Smad2 (L16D3) Mouse mAb.
Background
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
- Heldin, C.H. et al. (1997) Nature 390, 465-471.
- Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194.
- Derynck, R. et al. (1998) Cell 95, 737-740.
- Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791.
- Whitman, M. (1998) Genes Dev. 12, 2445-2462.
- Wu, G. et al. (2000) Science 287, 92-97.
- Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-1647.
- Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-4369.
Application References
- Li, Q. et al. (2008) Mol Cell Biol 28, 7001-11. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
