Product Pathways - Glucose Metabolism
HNF4α (E410) Antibody #3105
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 52 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
HNF4α (E410) Antibody detects endogenous levels of total HNF4α protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence around E410 of human HNF4α. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HepG2 cells, using HNF4α (E410) Antibody.
Background
Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5).
- Sladek, F.M. et al. (1990) Genes Dev. 4, 2353-2365.
- Yoon, J.C. et al. (2001) Nature 413, 131-138.
- Rhee, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4012-4017.
- Hayhurst, G.P. et al. (2001) Mol. Cell Biol. 21, 1393-1403.
- Ellard, S. and Colclough, K. (2006) Hum. Mutat. 27, 854-869.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
