Product Pathways - Cytoskeletal Signaling

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Phospho-VASP (Ser157) Antibody #3111

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P	H M R Mk GP	Endogenous	50	Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3111:

IHC / Paraffin, Western Blotting

Specificity / Sensitivity

Phospho-VASP (Ser157) Antibody detects endogenous levels of VASP only when phosphorylated at serine 157. The antibody may cross-react with the phosphorylated VASP homologue Mena.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser157 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

1. Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
2. Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
3. Machesky, L.M. et al. (2000) Cell 101, 685-688.
4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
5. Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
6. Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
7. Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.

Application References

• Howe, A. K. et al. (2002) Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion. J. Biol. Chem. 277 (41), 38121-38126. Applications: Western Blotting
• López De Jesús, M. et al. (2006) J Biol Chem 281, 21837-47. Applications: Western Blotting
• Wentworth, J.K. et al. (2006) Biochem J 393, 555-64. Applications: Western Blotting
• Chen, H. et al. (2008) J Biol Chem 283, 4439-47. Applications: Western Blotting
• Bilodeau, M.L. and Hamm, H.E. (2007) J Pharmacol Exp Ther 322, 778-88. Applications: Western Blotting
• Gonzalez, A.M. et al. (2008) J Biol Chem 283, 31849-60. Applications: Western Blotting
• Forrester, M.T. et al. (2011) Biotechniques 50, 41-5. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3112 VASP Antibody
• 3114 Phospho-VASP (Ser239) Antibody
• 9621 Phospho-(Ser/Thr) PKA Substrate Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 3132 VASP (9A2) Rabbit mAb
• 3120 VASP (A290) Antibody
• 9967 Actin Reorganization Antibody Sampler Kit

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.