Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

VASP Antibody #3112

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 46, 50 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VASP Antibody detects endogenous levels of total VASP protein. The antibody does not cross-react with VASP homologues, such as EVL and Mena.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser157 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated THP-1 cells, untreated and forskolin treated C6 cells, and untreated C2C12 and COS cells, using the VASP Antibody.

Background

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

  1. Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
  2. Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
  3. Machesky, L.M. et al. (2000) Cell 101, 685-688.
  4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
  5. Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
  6. Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
  7. Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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