Product Pathways - Glucose Metabolism
HNF4α (C11F12) Rabbit mAb #3113
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IHC-P IF-IC | H | Endogenous | 52 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
HNF4α (C11F12) Rabbit mAb detects endogenous levels of total HNF4α protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human HNF4α.
Western Blotting
Western blot analysis of extract from HepG2 cells using HNF4α (C11F12) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma using HNF4α (C11F12) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HepG2 cells in the presence of control peptide (left) or antigen specific peptide (right) using HNF4α (C11F12E8) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HepG2 (left) and HeLa cells (right) using HNF4α (C11F12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Background
Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5).
- Sladek, F.M. et al. (1990) Genes Dev. 4, 2353-2365.
- Yoon, J.C. et al. (2001) Nature 413, 131-138.
- Rhee, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4012-4017.
- Hayhurst, G.P. et al. (2001) Mol. Cell Biol. 21, 1393-1403.
- Ellard, S. and Colclough, K. (2006) Hum. Mutat. 27, 854-869.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
