Product Pathways - Cytoskeletal Signaling
Phospho-VASP (Ser239) Antibody #3114
|3114S||100 µl (10 western blots)||---||In Stock||---|
|3114P||40 µl (4 western blots)||---||In Stock||---|
|3114||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||48, 50||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-VASP (Ser239) Antibody detects endogenous levels of VASP only when phosphorylated at serine 239. The antibody does not cross-react with phosphorylated VASP homologues, such as Mena.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser239 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from NIH/3T3 and C6 cells, untreated, 8-Br-cGMP-treated, 8-Br-cAMP-treated or forskolin-treated as indicated, using Phospho-VASP (Ser239) Antibody.
Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).
- Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
- Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
- Machesky, L.M. et al. (2000) Cell 101, 685-688.
- Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
- Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
- Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
- Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.
- Chen, H. et al. (2008) J Biol Chem 283, 4439-47. Applications: Western Blotting.
- Negash, S. et al. (2009) Am J Physiol Heart Circ Physiol , . Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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