Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-VASP (Ser239) Antibody #3114

Applications Reactivity MW (kDa) Source
W H M R Mk 48, 51 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-VASP (Ser239) Antibody detects endogenous levels of VASP only when phosphorylated at serine 239. The antibody does not cross-react with phosphorylated VASP homologues, such as Mena.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser239 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells stimulated with forskolin for indicated times, using Phospho-VASP (Ser239) Antibody (upper) and VASP Antibody #3112 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and C6 cells, untreated, 8-Br-cGMP-treated, 8-Br-cAMP-treated or forskolin-treated as indicated, using Phospho-VASP (Ser239) Antibody.

Background

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

  1. Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
  2. Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
  3. Machesky, L.M. et al. (2000) Cell 101, 685-688.
  4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
  5. Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
  6. Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
  7. Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.

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