Cell Signaling Technology

Product Pathways - Metabolism

HNF4α (G162) Antibody #3117

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 52 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HNF4α (G162) Antibody detects endogenous levels of total HNF4α protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human HNF4α. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2 cells, using HNF4α (G162) Antibody.

Background

Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5).

  1. Sladek, F.M. et al. (1990) Genes Dev. 4, 2353-2365.
  2. Yoon, J.C. et al. (2001) Nature 413, 131-138.
  3. Rhee, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4012-4017.
  4. Hayhurst, G.P. et al. (2001) Mol. Cell Biol. 21, 1393-1403.
  5. Ellard, S. and Colclough, K. (2006) Hum. Mutat. 27, 854-869.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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