Product Pathways - Tyrosine Kinase / Adaptors

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Phospho-Met (Tyr1234/1235) Antibody #3126

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Mk	Endogenous	145	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3126:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Phospho-Met (Tyr1234/1235) Antibody detects endogenous levels of Met only when phosphorylated at tyrosine 1234/1235. This antibody cross-reacts with activated Ron, EGF, PDGF, insulin and FGF receptors.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1234/1235 of human Met. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).

1. Cooper, C.S. et al. (1984) Nature 311, 29-33.
2. Bottaro, D.P. et al. (1991) Science 251, 802-4.
3. Bardelli, A. et al. (1997) Oncogene 15, 3103-11.
4. Taher, T.E. et al. (2002) J Immunol 169, 3793-800.
5. Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32.
6. Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14.
7. Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118.

Application References

• Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7. Applications: Western Blotting
• Rikova, K. et al. (2007) Cell 131, 1190-203. Applications: Western Blotting
• Emaduddin, M. et al. (2008) Proc Natl Acad Sci U S A 105, 2358-62. Applications: Western Blotting
• Miyata, Y. et al. (2006) Clin Cancer Res 12, 4876-81. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3077 Phospho-Met (Tyr1234/1235) (D26) XP^® Rabbit mAb
• 3148 Met (L41G3) Mouse mAb
• 3127 Met (25H2) Mouse mAb
• 4560 Met Antibody
• 3129 Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb
• 3133 Phospho-Met (Tyr1349) (130H2) Rabbit mAb
• 3121 Phospho-Met (Tyr1349) Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7242 PathScan^® Total Met Sandwich ELISA Kit

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For Research Use Only. Not For Use In Diagnostic Procedures.