Product Pathways - Cytoskeletal Signaling
ARP2 Antibody #3128
PhosphoSitePlus® protein, site, and accession data: ARP2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Hm Mk Dm | Endogenous | 44 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3128:
- Western Blotting
Specificity / Sensitivity
ARP2 Antibody detects endogenous levels of total ARP2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ARP2.
Western Blotting
Western blot analysis of extracts from various cell types using ARP2 Antibody.
Background
Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3 and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).
- Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
- Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
- Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
