Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb #3129
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M R | 145 Kda | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb detects endogenous levels of Met only when phosphorylated at tyrosine 1234/1235. This antibody may cross-react with activated Ron and FGF receptors.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr1234/1235 of human Met.
Western Blotting
Western blot analysis of extracts of H4IIE cells untreated or treated with HGF, using Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb (upper) or Met (25H2) Mouse mAb #3127 (lower).
Western Blotting
Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).
Background
Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor), is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. Addition of a phosphate at cytoplasmic Tyr1003 is essential for ubiquitination and Met protein degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6).
- Weidner, K.M. et al. (1993) J. Cell Biol. 121, 145-154.
- Park, M. et al. (1986) Cell 45, 895-904.
- Bardelli, A. et al. (1997) Oncogene 15, 3103-3111.
- Taher, T.E. et al. (2002) J. Immunol. 169, 3793-3800.
- Schaeper, U. et al. (2000) J. Cell Biol. 149, 1419-1432.
- Traxler, P. et al. (2001) Med. Res. Rev. 21, 499-512.
Application References
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Companion Products
- 3121 Phospho-Met (Tyr1349) Antibody
- 3126 Phospho-Met (Tyr1234/1235) Antibody
- 3127 Met (25H2) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3133 Phospho-Met (Tyr1349) (130H2) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
