Product Pathways - Tyrosine Kinase/ Adaptors
Ack1 Antibody #3131
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 100, 135 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Ack1 Antibody detects endogenous levels of total Ack1 protein. This antibody may cross-react with Ack2 but not other related proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Pro90 of human Ack1. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of cell extracts from HEK293 cells, either mock-transfected or transfected with myc-tagged human Ack1 expression vector, using Ack1 Antibody (upper) or Myc-Tag Antibody #2272 (lower).
Western Blotting
Western blot analysis of cell extracts from various cell types using Ack1 Antibody.
Background
Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF, PDGF as well as by activation of integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).
- Galisteo, M.L. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9796-9801.
- Yokoyama, N. and Miller, W.T. (2003) J. Biol. Chem. 278, 47713-47723.
- Yang, W. and Cerione, R.A. (1997) J. Biol. Chem. 272, 24819-24824.
- Yang, W. et al. (2001) J. Biol. Chem. 276, 43987-43993.
- Mahajan, N.P. et al. (2005) Cancer Res. 65, 10514-10523.
Application References
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Companion Products
- 3138 Phospho-Ack1 (Tyr284) Antibody
- 3137 Phospho-Ack1 (Tyr857/858) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
