Product Pathways - Cytoskeletal Signaling
VASP (9A2) Rabbit mAb #3132
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk Hm B | 46, 50 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Hm=Hamster
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
VASP (9A2) Rabbit mAb detects endogenous levels of total VASP protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide corresponding to residues near the carboxy terminus of human and mouse VASP.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, COS, Cho and C6 cells using VASP (9A2) Rabbit mAb.
IP
Immunoprecipitation of VASP from HeLa cell extracts using VASP Rabbit mAb. The same antibody was used for Western detection.
IF-IC
Confocal immunofluorescent analysis of HUVE cells using VASP (9A2) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).
- Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
- Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
- Machesky, L.M. et al. (2000) Cell 101, 685-688.
- Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
- Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
- Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
- Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3111 Phospho-VASP (Ser157) Antibody
- 3114 Phospho-VASP (Ser239) Antibody
- 3120 VASP (A290) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
