Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

VASP (9A2) Rabbit mAb #3132

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Hm Mk B Endogenous 46, 50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VASP (9A2) Rabbit mAb detects endogenous levels of total VASP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human and mouse VASP.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VASP (9A2) Rabbit mAb.

IP

IP

Immunoprecipitation of VASP from HeLa cell extracts using VASP Rabbit mAb. The same antibody was used for Western detection.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HUVE cells using VASP (9A2) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

  1. Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
  2. Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
  3. Machesky, L.M. et al. (2000) Cell 101, 685-688.
  4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
  5. Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
  6. Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
  7. Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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