Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Actinin Antibody #3134

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Hm Endogenous 100 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

α-Actinin Antibody detects endogenous levels of total α-actinin-1 protein. Based on sequence homology, the antibody may cross-react with α-actinin isoforms 2 and 4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human α-actinin-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, C6, CHO and COS cells using α-Actinin Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells using alpha-Actinin Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

α-actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, α-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration and immune cell targeting. (1). The interaction of α-actinin with ICAM5 (intercellular adhesion molecule-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of α-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).

  1. Otey, C.A. and Carpen, O. (2004) Cell Motil. Cytoskeleton 58, 104-111.
  2. Nyman-Huttunen, H. et al. (2006) J. Cell Sci. 119, 3057-3066.
  3. Triplett, J.W. and Pavalko, F.M. (2006) Am. J. Physiol. Cell Physiol. 291, C909-921.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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