Product Pathways - Cell Cycle / Checkpoint
CDK6 (DCS83) Mouse mAb #3136
|3136S||100 µl (20 western blots)||---||In Stock||---|
|3136P||40 µl (8 western blots)||---||In Stock||---|
|3136||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:2000||Human, Mouse, Rat||Endogenous||36||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
CDK6 (DCS83) Mouse mAb detects endogenous levels of total CDK6 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CDK6 corresponding to amino acids 30-65.
The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of CDK inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4).
- Lukas, C. et al. (1999) Hybridoma 18 (3), 225-234. Applications: Western Blotting, IC-IF.
- Chang, S.F. et al. (2008) Proc Natl Acad Sci U S A 105, 3927-32. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.