Product Pathways - Cell Cycle / Checkpoint
CDK6 (DCS83) Mouse mAb #3136
PhosphoSitePlus® protein, site, and accession data: CDK6
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R | Endogenous | 36 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3136:
- Western Blotting
Specificity / Sensitivity
CDK6 (DCS83) Mouse mAb detects endogenous levels of total CDK6 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CDK6 corresponding to amino acids 30-65.
Western Blotting
Western blot analysis of extracts from HeLa, IM-CD-3 and C6 cells, using CDK6 (DCS83) Mouse mAb.
Background
The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of cdk inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4).
- Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.
- Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
- Pestell, R.G. et al. (1999) Endocr. Rev. 20, 501-534.
- Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.
Application References
- Lukas, C. et al. (1999) Immunochemical analysis of the D-type cyclins-dependent kinases cdk4 and cdk6, using a series of monoclonal antibodies. Hybridoma 18 (3), 225-234. Applications: IC-IF Western Blotting
- Chang, S.F. et al. (2008) Proc Natl Acad Sci U S A 105, 3927-32. Applications: Western Blotting
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
