Product Pathways - Cell Cycle / Checkpoint
CDK6 (DCS83) Mouse mAb #3136
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M R | 36 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CDK6 (DCS83) Mouse mAb detects endogenous levels of total CDK6 protein.
Source / Purification
Monoclonal antibody is produced by immunizing mice with recombinant human CDK6 corresponding to amino acids 30-65.
Western Blotting
Western blot analysis of extracts from HeLa, IM-CD-3 and C6 cells, using CDK6 (DCS83) Mouse mAb.
Background
The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of cdk inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4).
- Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.
- Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
- Pestell, R.G. et al. (1999) Endocr. Rev. 20, 501-534.
- Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.
Application References
- Lukas, C. et al. (1999) Immunochemical analysis of the D-type cyclins-dependent kinases cdk4 and cdk6, using a series of monoclonal antibodies. Hybridoma 18 (3), 225-234. This article references the use of CDK6 (DCS83) Mouse mAb in the following applications: IC-IF Western Blotting
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