Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-Ack1 (Tyr857/858) Antibody #3137
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M | 135 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Ack1 (Tyr857/858) Antibody detects overexpressed levels of Ack1 only when phosphorylated at Tyr857/858. This antibody does not cross-react with other tyrosine-phosphorylated tyrosine kinases.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr857/858 of human Ack1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using Phospho-Ack1 (Tyr857/858) Antibody (upper and middle), and Myc-tag (71D10) Antibody #2278 (lower). The middle blot was treated with phosphatase before antibody probing. The lysate of COS cells overexpressing Myc-tagged Ack1 protein was kindly provided by Dr. Wannian Yang.
Background
Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF, PDGF as well as by activation of integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).
Phosphorylation of Ack1 on Tyr857 and Tyr858 on Ack1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery as well as other publications using MS technology (5,6). Phosphorylation of Ack1 at Tyr857/858 was observed in select carcinoma cell lines and in tumors.
- Galisteo, M.L. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9796-9801.
- Yokoyama, N. and Miller, W.T. (2003) J. Biol. Chem. 278, 47713-47723.
- Yang, W. and Cerione, R.A. (1997) J. Biol. Chem. 272, 24819-24824.
- Yang, W. et al. (2001) J. Biol. Chem. 276, 43987-43993.
- Mahajan, N.P. et al. (2005) Cancer Res. 65, 10514-10523.
- Zhang, Y. et al. (2005) Mol. Cell Proteomics 4, 1240-1250.
- Salomon, A.R. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 443-448.
Application References
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