Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Ezrin/Radixin/Moesin Antibody #3142

Applications Reactivity MW (kDa) Source
W H M R Mk B 75 Moesin. 80 Ezrin and Radixin. Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Ezrin/Radixin/Moesin Antibody detects endogenous levels of ezrin, radixin and moesin. The antibody does not cross-react with related proteins such as merlin or band 4.1.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to the residues surrounding Thr567 of human ezrin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, L929, C6 and COS cells, using Ezrin/Radixin/Moesin Antibody.

Background

The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts their amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

  1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
  2. Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
  3. Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
  4. Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
  5. Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
  6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes