Product Pathways - Cytoskeletal Signaling
Ezrin/Radixin/Moesin Antibody #3142
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R Mk B (X) | Endogenous | 75 Moesin. 80 Ezrin and Radixin. | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
X=Xenopus
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3142:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
Ezrin/Radixin/Moesin Antibody detects endogenous levels of ezrin, radixin and moesin. The antibody does not cross-react with related proteins such as merlin or band 4.1.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Thr567 of human ezrin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using Ezrin/Radixin/Moesin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ezrin/Radixin/Moesin Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Ezrin/Radixin/Moesin Antibody.
Background
The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
- Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
- Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
- Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
- Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
- Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
- Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.
Application References
- Shi-Wen, X. et al. (2004) Endothelin-1 Promotes Myofibroblast Induction through the ETA Receptor via a rac/Phosphoinositide 3-Kinase/Akt-dependent Pathway and Is Essential for the Enhanced Contractile Phenotype of Fibrotic Fibroblasts. Mol. Biol. Cell 15, 2707-2719. Applications: Western Blotting
- John, G. R. et al. (2004) Interleukin-1beta Induces a Reactive Astroglial Phenotype via Deactivation of the Rho GTPase-Rock Axis. J. Neurosci. 21 (11), 2837-2845. Applications: Western Blotting
- Duterme, C. et al. (2009) J Biol Chem 284, 33495-508. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3141 Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3145 Ezrin Antibody
- 3144 Phospho-Ezrin (Tyr353) Antibody
- 3146 Moesin Antibody
- 3150 Moesin (Q480) Antibody
- 3149 Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb
- 8112 SignalStain® Antibody Diluent
For Research Use Only. Not For Use In Diagnostic Procedures.
