Cell Signaling Technology

Product Pathways - Metabolism

FoxA2/HNF3β Antibody #3143

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H (M) (R) Endogenous 50 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FoxA2/HNF3β Antibody detects endogenous levels of total FoxA2/HNF3β protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human FoxA2/HNF3β. Antibodies are purified by Protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2 cells using FoxA2/HNF3β Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HepG2 cells using FoxA2/HNF3β Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

Forkhead box protein A2 (FoxA2, also known as hepatocyte nuclear factor 3β or HNF3β) is a transcription factor that plays an important role in hepatocyte function (1). FoxA2/HNF3β is required for the activation of hepatic gluconeogenic gene expression during fasting (1). Together with the PGC-1β coactivator, FoxA2/HNF3β stimulates the expression of genes involved in fatty acid β-oxidation and therefore increases fatty acid metabolism (2). FoxA2/HNF3β, along with PGC-1β, also activates the expression of microsomal triacylglycerol transfer protein (MTP) and promotes VLDL secretion (2). In addition to its roles in metabolic syndromes, FoxA2/HNF3β is essential for development of the endoderm and midline structures in mouse embryos (3-5).

  1. Zhang, L. et al. (2005) Cell Metab 2, 141-8.
  2. Wolfrum, C. and Stoffel, M. (2006) Cell Metab 3, 99-110.
  3. Levinson-Dushnik, M. and Benvenisty, N. (1997) Mol Cell Biol 17, 3817-22.
  4. Weinstein, D.C. et al. (1994) Cell 78, 575-88.
  5. Ang, S.L. and Rossant, J. (1994) Cell 78, 561-74.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products