Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Ezrin (Tyr353) Antibody #3144

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Ezrin (Tyr353) Antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 353. The antibody does not cross-react with phosphorylated Moesin or Radixin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr353 of human Ezrin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or EGF-treated, using Phospho-Ezrin (Tyr353) Antibody (upper) or Ezrin/Radixin/Moesin Antibody #3142 (lower).

Background

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

  1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
  2. Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
  3. Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
  4. Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
  5. Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
  6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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