Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Ezh2 (AC22) Mouse mAb #3147

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk Endogenous 98 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ezh2 (AC22) Mouse mAb detects endogenous levels of total Ezh2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with Ezh2 recombinant protein containing human Ezh2 residues 351-453.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ezh2 (AC22) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Ezh2 (AC22) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

  1. Seller, W.B. and Loda, M. (2002) Cancer Cell 2, 349-350.
  2. Visser, H.P. et al. (2001) Br. J. Haematol. 112, 950-958.
  3. Chen, H. et al. (1996) Genomics 38, 30-37.
  4. Tonini, T. et al. (2004) Oncogene 23, 4930-4937.
  5. Muller, J. et al. (2002) Cell 111, 197-208.
  6. Kleer, C.G. et al. (2003) Proc Natl. Acad. Sci. USA 100, 11606-11611.

Application References

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Companion Products

Rabbit Monoclonals Produced UsingTechnology from Epitomics, Inc. UnderU.S. patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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