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Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P IF-IC	H M R Mk Dm B (X) (Dg)	Endogenous	75 Moesin. 80 Ezrin, Radixin.	Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster  X=Xenopus  B=Bovine  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3149:

IHC / Paraffin, Immunofluorescence, Western Blotting

Specificity / Sensitivity

Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb detects endogenous levels of ezrin, radixin and moesin only when phosphorylated at Thr567, 564 or 558, respectively. This antibody does not cross-react with related phospho-proteins such as merlin or band 4.1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin.

Background

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
2. Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
3. Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
4. Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
5. Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.

Application References

• Lavialle, M. et al. (2011) Proc Natl Acad Sci U S A 108, 12915-9. Applications: IF-IC (In Cells)
• Wakayama, Y. et al. (2011) J Biol Chem 286, 44243-53. Applications: IF-IC (In Cells) Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 3144 Phospho-Ezrin (Tyr353) Antibody
• 3141 Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody
• 3145 Ezrin Antibody
• 3142 Ezrin/Radixin/Moesin Antibody
• 3146 Moesin Antibody
• 3150 Moesin (Q480) Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 1047 Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) Blocking Peptide
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.