Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC H M R Mk Dm B (X) (Dg) Endogenous 75 Moesin. 80 Ezrin, Radixin. Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dm=D. melanogaster  X=Xenopus  B=Bovine  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb detects endogenous levels of ezrin, radixin and moesin only when phosphorylated at Thr567, 564 or 558, respectively. This antibody does not cross-react with related phospho-proteins such as merlin or band 4.1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin.

Western Blotting

Western Blotting

Western blot analysis of various cell extracts, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of untreated or λ phosphatase treated A431 cells using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb (upper) and Ezrin/Radixin/Moesin Antibody #3142 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149 in the presence of control peptide (left) or Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Blocking Peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb in the presence of control peptide (left) or Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) Blocking Peptide #1047 (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human stomach carcinoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HUVE cells, untreated (left) or TNF-α-treated (#8902, right), using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

  1. Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
  2. Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
  3. Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
  4. Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
  5. Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
  6. Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.


For Research Use Only. Not For Use In Diagnostic Procedures.

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