Product Pathways - Cytoskeletal Signaling
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC | H M R Mk B Dm | 75 Moesin. 80 Ezrin, Radixin. | Rabbit | IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Dm=D. melanogaster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb detects endogenous levels of ezrin, radixin and moesin only when phosphorylated at Thr567, 564 or 558, respectively. This antibody does not cross-react with related phospho-proteins such as merlin or band 4.1.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surroundingThr567 of human ezrin.
Western Blotting
Western blot analysis of various cell extracts, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb.
Western Blotting
Western blot analysis of untreated or λ phosphatase treated A431 cells using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb (upper) and Ezrin/Radixin/Moesin Antibody #3142 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149 in the presence of control peptide (left) or Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Blocking Peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb in the presence of control peptide (left) or Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) Blocking Peptide #1047 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma, using Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) (41A3) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells serum-starved (left) or λ phosphatase-treated (right), using Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts their amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
- Tsukita, S. and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510.
- Mangeat, P. et al. (1999) Trends Cell Biol. 9, 187-192.
- Matsui, T. et al. (1998) J. Cell Biol. 140, 647-657.
- Gautreau, A. et al. (2000) J. Cell Biol. 150, 193-203.
- Tran Quang, C. et al. (2000) EMBO J. 19, 4565-4576.
- Gautreau, A. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 7300-7305.
Application References
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Companion Products
- 3144 Phospho-Ezrin (Tyr353) Antibody
- 3141 Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody
- 3145 Ezrin Antibody
- 3142 Ezrin/Radixin/Moesin Antibody
- 3146 Moesin Antibody
- 3150 Moesin (Q480) Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 1047 Phospho-Ezrin (Thr567)/Radixin (Thr564)/ Moesin (Thr558) Blocking Peptide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
