Product Pathways - Nuclear Receptor Signaling
Progesterone Receptor A/B (C89F7) Rabbit mAb #3153
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P F | H | Endogenous | 90 (PR-A) and 118 (PR-B) | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 3153:
- Flow, IHC / Paraffin, Western Blotting
Specificity / Sensitivity
Progesterone Receptor A/B (C89F7) Rabbit mAb detects endogenous levels of total progesterone receptor A and B proteins. This antibody does not cross-react with other PR family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr541 of human progesterone receptor.
Western Blotting
Western blot analysis of extracts from T47D cells using Progesterone Receptor A/B (C89F7) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of T-47D cells using Progesterone Receptor A/B (C89F7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Progesterone Receptor A/B (C89F1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded T47D cells (positive, left) and MDA-MB-231 cells (negative, right) using Progesterone Receptor A/B (C89F1) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Progesterone Receptor A/B (C89F1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Human progesterone receptor (PR) is expressed as two forms: the full length PR B and the short form PR A. PR A lacks the first 164 amino acid residues of PR B (1,2). Both PR A and PR B are ligand activated but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102 and Ser162) are unique to full length PR B while other sites (Ser190, Ser294, Ser345 and Ser400) are shared by both isoforms (5). Phosphorylation of PR B at Ser190 (equivalent to Ser26 of PR A) is catalyzed by CDK2 (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation of Ser190 may be critical to its biological function.
- Evans, R.M. (1988) Science 240, 889-895.
- Kastner, P. et al. (1990) EMBO J. 112, 1603-1614.
- Giangrande, P.H. et al. (2000) Mol. Cell. Biol. 20, 3102-3115.
- Wen, D.X. et al. (1994) Mol. Cell. Biol. 14, 8356-8364.
- Clemm, D.L. et al. (2000) Mol. Endocrinol. 14, 52-65.
- Zhang, Y. et al. (1997) Mol. Endocrinol. 11, 823-832.
- Takimoto, G.S. et al. (1996) J. Biol. Chem. 271, 13308-13316.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3171 Phospho-Progesterone Receptor (Ser190) Antibody
- 3172 Progesterone Receptor (6A1) Mouse mAb
- 3176 Progesterone Receptor A/B Antibody
- 3178 Progesterone Receptor B Antibody
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3157 Progesterone Receptor B (C1A2) Rabbit mAb
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
