Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb #3155

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P F H M Endogenous 175 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb detects endogenous levels of M-CSF receptor only when phosphorylated at tyrosine 723. The antibody does not cross-react with related active protein tyrosine kinases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr723 of human M-CSF receptor.

Western Blotting

Western Blotting

Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb specifically binds to phosphorylated M-CSF receptors, but not other phosphorylated protein tyrosine kinases. Western blot anlysis of extracts from various cell lines expressing different activated protein tyrosine kinases, using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb (upper). The same membrane was stripped and reprobed with Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from NKM-1 cells, untreated or treated with a M-CSFR inhibitor, using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb (upper) or M-CSF Receptor Antibody #3152 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded FDCP1/fms cells, untreated (left) or mCSF-treated (right), using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ba/F3-CSF-1R cells, untreated (blue) or mCSF-treated (green), using Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb compared to a nonspecific negative control antibody (red).

Background

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLC-γ 2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).

  1. Stanley, E. R. et al. (1978) Nature 274, 168-170.
  2. Byrne, P. V. et al. (1981) J. Cell. Biol. 91, 848-853.
  3. Bourette, R. P. et al. (2000) Growth Factors 17, 155-166.
  4. Novak, U. et al. (1996) Oncogene 13, 2607-2613.
  5. Bourette, R. P. et al. (1997) EMBO J. 16, 5880-5893.
  6. Morley, G. M. et al. (1999) Oncogene 18, 3076-3084.
  7. Toy, E. P. et al. (2001) Gynecol. Oncol. 80, 194-200.
  8. Maher, M. G. et al. (1998) Clin. Cancer Res. 4, 1851-1856.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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