Product Pathways - Nuclear Receptor Signaling
Progesterone Receptor B (C1A2) Rabbit mAb #3157
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 118 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Progesterone Receptor B (C1A2) Rabbit mAb detects endogenous levels of total progesterone receptor B protein. This antibody does not cross-react with other PR family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser115 of human progesterone receptor.
Western Blotting
Western blot analysis of extracts from MCF-7 and T47D cells using Progesterone Receptor B (C1A2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Progesterone Receptor B Receptor (C1A2) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow Cytometry
Flow cytometric analysis of T-47D cells using Progesterone Receptor B Receptor (C1A2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using Progesterone Receptor B Receptor (C1A2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Human progesterone receptor (PR) is expressed as two forms: the full length PR B and the short form PR A. PR A lacks the first 164 amino acid residues of PR B (1,2). Both PR A and PR B are ligand activated but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102 and Ser162) are unique to full length PR B while other sites (Ser190, Ser294, Ser345 and Ser400) are shared by both isoforms (5). Phosphorylation of PR B at Ser190 (equivalent to Ser26 of PR A) is catalyzed by CDK2 (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation of Ser190 may be critical to its biological function.
- Evans, R.M. (1988) Science 240, 889-895.
- Kastner, P. et al. (1990) EMBO J. 112, 1603-1614.
- Giangrande, P.H. et al. (2000) Mol. Cell. Biol. 20, 3102-3115.
- Wen, D.X. et al. (1994) Mol. Cell. Biol. 14, 8356-8364.
- Clemm, D.L. et al. (2000) Mol. Endocrinol. 14, 52-65.
- Zhang, Y. et al. (1997) Mol. Endocrinol. 11, 823-832.
- Takimoto, G.S. et al. (1996) J. Biol. Chem. 271, 13308-13316.
Application References
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3153 Progesterone Receptor A/B (C89F7) Rabbit mAb
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
