Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

IFN-γ (3F1E3) Mouse mAb #3159

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP E-P H Recombinant 17 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  E-P=ELISA (Peptide)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IFN-γ (3F1E3) Mouse mAb detects recombinant human IFN-γ.

Source / Purification

Monoclonal antibody is produced by immunizing animals with Ni-NTA purified recombinant human IFN-γ expressed in E. Coli. Antibodies were prepared from ascites.

Western Blotting

Western Blotting

Western blot analysis of lysates containing recombinant human IFN-γ using IFN-γ (3F1E3) Mouse mAb.

ELISA-Peptide

ELISA-Peptide

ELISA analysis of plates coated with recombinant human IFN-γ using IFN-γ (3F1E3) Mouse mAb.

Background

Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation (1). There are three naturally occurring interferons: α, β and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2). IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε) (3). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.

IFN-γ, also known as type II interferon, is produced mainly in activated T lymphocytes and natural killer cells (4) and has broad effects on various cells of the immune system. Many signaling proteins including IL-2, FGF, and EGF induce the synthesis of IFN-γ.

  1. Stiehm, E.R. et al. (1982) Ann Intern Med 96, 80-93.
  2. Pellegrini, S. et al. (1989) Mol Cell Biol 9, 4605-12.
  3. Pfeffer, L.M. and Colamonici, O.R. (1991) Pharmacol Ther 52, 149-57.
  4. Young, H.A. and Hardy, K.J. (1995) J Leukoc Biol 58, 373-81.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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