Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

Zap-70 (D1C10E) XP® Rabbit mAb #3165

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-F F H M Endogenous 70 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Zap-70 (D1C10E) XP® Rabbit mAb detects endogenous levels of total Zap-70. This antibody does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human Zap-70.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells using Zap-70 (D1C10E) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos B cells (red) and Jurkat T cells (blue), using Zap-70 (D1C10E) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of human tonsil using Zap-70 (D1C10E) XP® Rabbit mAb (red) showing colocalization with T-cells that have been labeled with CD3 Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

  1. Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
  2. Iwashima, M. et al. (1994) Science 263, 1136-1139.
  3. Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
  4. Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
  5. Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
  6. Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
  7. Wiestner, A. et al. (2003) Blood 101, 4944-4951.
  8. Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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