Product Pathways - Nuclear Receptor Signaling
Progesterone Receptor (6A1) Mouse mAb #3172
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IC-ABC | H | 90 (PR-A). 118 (PR-B). | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IC-ABC=Immunocytochemistry (ABC)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Progesterone Receptor (6A1) Monoclonal Antibody detects endogenous levels of total progesterone receptor. It does not cross-react with other PR family members.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser190 of human progesterone receptor.
Western Blotting
Western blot analysis of extracts from T47D cells, untreated or stimulated with 100 nM promegestone (R5020) for 1 hour, using Progesterone Receptor (6A1) Mouse Monoclonal mAb.
Background
Human progesterone receptor (PR) is expressed as two forms: the full length PR B and the short form PR A. PR A lacks the first 164 amino acid residues of PR B (1,2). Both PR A and PR B are ligand activated but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102 and Ser162) are unique to full length PR B while other sites (Ser190, Ser294, Ser345 and Ser400) are shared by both isoforms (5). Phosphorylation of PR B at Ser190 (equivalent to Ser26 of PR A) is catalyzed by CDK2 (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation of Ser190 may be critical to its biological function.
- Evans, R.M. (1988) Science 240, 889-895.
- Kastner, P. et al. (1990) EMBO J. 112, 1603-1614.
- Giangrande, P.H. et al. (2000) Mol. Cell. Biol. 20, 3102-3115.
- Wen, D.X. et al. (1994) Mol. Cell. Biol. 14, 8356-8364.
- Clemm, D.L. et al. (2000) Mol. Endocrinol. 14, 52-65.
- Zhang, Y. et al. (1997) Mol. Endocrinol. 11, 823-832.
- Takimoto, G.S. et al. (1996) J. Biol. Chem. 271, 13308-13316.
Application References
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