Product Pathways - Translational Control
BiP (C50B12) Rabbit mAb #3177
|W IHC-P IHC-F||H M||Endogenous||78||Rabbit IgG|
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
BiP (C50B12) Rabbit mAb detects endogenous levels of total BiP protein.
Source / Purification
BiP (C50B12) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gly584 of human BiP.
Western blot analysis of extracts from various cell lines using BiP (C50B12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human glioblastoma using BiP (C50B12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using BiP (C50B12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using BiP (C50B12) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using BiP (C50B12) Rabbit mAb in the presence of control peptide (left) or BiP Blocking Peptide #1084 (right).
Immunohistochemical analysis of frozen SKOV-3 xenograft using BiP (C50B12) Rabbit mAb.
Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).
- Wabl, M. and Steinberg, C. (1982) Proc. Natl. Acad. Sci. USA 79, 6976-6978.
- Haas, I.G. and Wabl, M. Nature 306, 387-389.
- Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300.
- Kohno, K. et al. (1993) Mol. Cell Biol. 13, 877-890.
- Guo, L. et al. (2011) J Biol Chem 286, 18170-80. Applications: Western Blotting
- Moumen, A. et al. (2011) PLoS One 6, e26066. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.