Product Pathways - Translational Control
Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179
PhosphoSitePlus® protein, site, and accession data: PERK
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | R (M) | Endogenous | 170 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3179:
- Western Blotting
Specificity / Sensitivity
Phospho-PERK (Thr980) (16F8) Rabbit mAb detects endogenous levels of PERK phosphorylated at Thr980.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr980 of mouse PERK.
Western Blotting
Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.
Background
Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.
- Harding, H. et al. (1999) Nature 397, 271-274.
- Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
- Harding, H. et al. (2000) Mol. Cell 5, 897-904.
- Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.
Application References
- Chen, C.H. et al. (2011) Sci Signal 4, ra10. Applications: Western Blotting
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
