Product Pathways - Translational Control
Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | R (M) | Endogenous | 170 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 3179:
- Western Blotting
Specificity / Sensitivity
Phospho-PERK (Thr980) (16F8) Rabbit mAb detects endogenous levels of PERK phosphorylated at Thr980.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr980 of mouse PERK.
Western Blotting
Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.
Background
PERK (protein kinase-like endoplasmic reticulum kinase) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.
- Harding, H. et al. (1999) Nature 397, 271-274.
- Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
- Harding, H. et al. (2000) Mol. Cell 5, 897-904.
- Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
