Product Pathways - Translational Control
Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | R (M) | 170 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-PERK (Thr980) (16F8) Rabbit mAb detects endogenous levels of PERK phosphorylated at Thr980.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr980 of mouse PERK.
Western Blotting
Western blot analysis of extracts from AR42J cells untreated (-) or treated with 1 μM thapsigargin (Tg) for 20 minutes (+), using Phospho-PERK (Thr980) (16F8) Rabbit mAb.
Background
PERK, an eIF2α kinase, is a transmembrane protein resident in the endoplasmic reticulum (ER) membrane and couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α, resulting in reduced translation. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 can serve as a marker for its activation status.
- Harding, H. et al. (1999) Nature 397, 271-274.
- Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
- Harding, H. et al. (2000) Mol. Cell 5, 897-904.
- Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
