Cell Signaling Technology

Product Pathways - Glucose Metabolism

Fatty Acid Synthase (C20G5) Rabbit mAb #3180

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IHC-F IF-IC H M R (B) Endogenous 273 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Fatty Acid Synthase (C20G5) Rabbit mAb detects endogenous levels of total fatty acid synthase protein.

Source / Purification

Fatty Acid Synthase (C20G5) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) around Gly46 derived from the sequence of human fatty acid synthase.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Fatty Acid Synthase (C20G5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded breast carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lung carcinoma using Fatty Acid Synthase (C20G5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lymphoma, showing staining of adipocytes, using Fatty Acid Synthase (C20G5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brown fat using Fatty Acid Synthase (C20G5) Rabbit mAb.


IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen SKOV-3 xenograft using Fatty Acid Synthase (C20G5) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Fatty Acid Synthase (C20G5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Fatty acid synthase (FAS) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FAS is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FAS is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1).More recently, increased expression of FAS has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FAS are directly related to the size of breast tumors (2). Studies also showed that FAS is highly expressed in lung and prostate cancers and that FAS expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FAS is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FAS as a potential target for the diagnosis and treatment of cancer as well as the metabolic syndrome (6,7).

  1. Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
  2. Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40.
  3. Kawamura, T. et al. (2005) Pathobiology 72, 233-240.
  4. Shah, U.S. et al. (2006) Hum Pathol 37, 401-409.
  5. Kuhajda, F.P. (2000) Nutrition 16, 202-8.
  6. Tian, W.X. (2006) Curr Med Chem 13, 967-977.
  7. Kusunoki, J. et al. (2006) Endocrine 29, 91-100.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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