Product Pathways - Translational Control
BiP Antibody #3183
|W||H M R Mk||Endogenous||78||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
BiP Antibody detects endogenous levels of total BiP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human BiP. Antibodies are purified by protein A and peptide affinity chromatography.
Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).
- Wabl, M. and Steinberg, C. (1982) Proc. Natl. Acad. Sci. USA 79, 6976-6978.
- Haas, I.G. and Wabl, M. Nature 306, 387-389.
- Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300.
- Kohno, K. et al. (1993) Mol. Cell Biol. 13, 877-890.
- De Raedt, T. et al. (2011) Cancer Cell 20, 400-13. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.