Cell Signaling Technology

Product Pathways - Translational Control

BiP Antibody #3183

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 78 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BiP Antibody detects endogenous levels of total BiP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human BiP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from several cell lines using BiP Antibody.

Background

Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).

  1. Wabl, M. and Steinberg, C. (1982) Proc. Natl. Acad. Sci. USA 79, 6976-6978.
  2. Haas, I.G. and Wabl, M. Nature 306, 387-389.
  3. Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300.
  4. Kohno, K. et al. (1993) Mol. Cell Biol. 13, 877-890.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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