Product Pathways - Glucose Metabolism
Synip Antibody #3185
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M | Endogenous | 62 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Synip Antibody detects endogenous levels of total Synip protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human Synip. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extract from RD cells, using Synip Antibody.
Background
Insulin binds to and activates its receptor and initiates a signaling cascade that eventually induces the translocation of the Glut4 glucose transporter from its intracellular locations to the plasma membrane. Initiating this pathway facilitates glucose uptake in fat and skeletal muscle cells (1). Synip and Syntaxin 4 are two proteins thought to be involved in the recruitment of Glut4-containing vesicles to plasma membrane (2,3). Synip associates with Syntaxin 4 when insulin is absent. Insulin signaling triggers the dissociation of the two proteins and allows Syntaxin 4 to complex with VAMP2, which is essential for Glut4 translocation to plasma membrane (2-4). Overexpression of a dominant-negative form of Synip prevents Glut4 from translocating to plasma membrane in response to insulin stimulation (3). Synip together with Syntaxin 4, therefore, regulates Glut 4 transport to plasma membrane.
- Watson, R.T. and Pessin, J.E. (2006) Trends Biochem. Sci. 31, 215-222.
- Min, J. et al. (1999) Mol. Cell 3, 751-760.
- Yamada, E. et al. (2005) J. Cell Biol. 168, 921-928.
- Foster, L.J. and Klip, A. (2000) Am. J. Physiol. Cell Physiol. 279, C877-C890.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
